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. 2022 Nov 4;16:30. doi: 10.1186/s13036-022-00309-5

Table 1.

The implementation conditions and transfection results of nucleofection in related studies

Cell type Species Passage Cell confluence Procedure Cell viability Transfection efficiency Reference
Dermal fibroblasts Rat 2 80%-90% U-30  > 95.00%

·51.50% ± 7.90%

(Standard method)

·80.50% ± 5.00%

(Modified method: DMEM + 10% FCS)

 [11]
Dermal fibroblasts Rat  ≤ 3 80%-90% U-30 /

·68.34% ± 10.32% (Standard method)

·85.35% ± 11.56%

(Modified method: DMEM + 10% FCS)

 [13]
Dermal fibroblasts Human  ≤ 3 80%-90% U-24 /

·83.88% ± 9.67%

(Standard method)

·57.88% ± 3.45%

(Modified method: DMEM + 10% FCS)

·79.21% ± 1.62%

(Modified method:

ITS supplement)

 [13]
Dermal fibroblasts Human / 80%-90% U-20

80.00%-

90.00%

 40.00%-50.00% [14]
Dermal fibroblasts Pig 3–4 / V-13 / 60.10% [19]
Dermal fibroblasts Rat 2–4 / U-23 91.10% 72.50% [20]
Dermal fibroblasts Human / / U-23 37.00% 10.00% [23]
Dermal fibroblasts Human 1–5 /

U-30

T-018

  > 68.70% 34.60% [28]
Dermal fibroblasts Pig / 90%

U-23

V-13

87.90% ± 

7.40%

(U23)/

85.40% ± 

7.50%

(V13)

·80.80% ± 6.20%

(U23)

·86.00% ± 2.50%

(V13)

 [30]
Sciatic nerve fibroblasts Rat / 80% T-16 / 80.00% [31]
Dermal fibroblasts Rat / /

U-23

P-22

 /

·57.33% (U-23)

·57.00% (P-22)

 [33]
Embryonic fibroblasts Zebrafish / / O-20

30.00%-

35.00%

 35.00%-43.00% [35]
Fetal fibroblast Buffalo of Murrah breed / 70%-80% EN-150

70.00%-

80.00%

 73.60% ± 1.36% [36]
Fetal fibroblast Buffalo of Murrah breed 2–3 70%-80% EN-150

53.80% ± 

4.20%

 73.50% ± 1.40% [41]
Embryo fibroblasts Pig 16 / U-20 95.00% 90.00% [46]
Embryonic ear fibroblasts Rabbit / / U-23 / 38.00% [46]
Embryonic fibroblasts Pig 3–7 / U-23 57.80% 79.00% ± 0.80% [48]
Kidney fibroblasts Pig / / T-007 / 65.00%-70.00% [49]