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. 2022 Sep 22;33(12):ar115. doi: 10.1091/mbc.E22-04-0118

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FIGURE 11: — Immunodepletion of the four major mitotic kinases had no or minor effects on MEE-induced supershifts of the five supershift fragments. (A) Immunodepletion of MEE with each of indicated kinase-specific antibodies or control IgG, followed by immunoblotting of SNs with antibodies for each of indicated proteins. (B) Phosphorylation of each of myc-tagged supershift fragments with individual kinase-depleted or control IgG-absorbed MEE for the indicated minutes, followed by myc-tag immunoblotting. (C) Phosphorylation of each of myc-tagged supershift fragments with all four kinase-depleted or control IgG-absorbed MEE for the indicated minutes, followed by myc-tag immunoblotting.