FIGURE 5:

Loss of AP-4 in i³Neurons results in accumulation of JIP3 in axonal swellings. (A and B) Immunoblotting reveals increased levels of JIP3 in AP-4 i³Neurons compared with Control i³Neurons (21 DIV; ACTB used as loading Control; mean ± SEM from three independent experiments; **P < 0.01; ***P < 0.001). (C) Representative images of Control and AP-4 G4i i³Neurons (28 DIV) stained for JIP3 (green) and MAP2 (blue). Arrowheads highlight JIP3 accumulation in the axons of AP-4 G4i i³Neurons. Bar, 20 µm. (D) Quantification of axonal JIP3-positive swellings per unit area in AP-4 i³Neurons compared with Control i³Neurons (28 DIV; mean ± SEM from three independent experiments; > 20 areas chosen at random per genotype; *P < 0.05).