FIGURE 4:

A proportion of glucose release follows a canonical glucose-6-phosphate translocase and glucose-6-phosphate-phosphatase route. HAMEC were pulsed with [³H]-2-DG as in Figure 2. This was followed by 30-min (A and B) or 15-min (C) chase periods at 37°C that included (A) 10 µM glucose-6-phosphate translocase inhibitor, S4048 vs. control (0.1% vol/vol DMSO); (B) 100 µM glucose-6-phosphatase inhibitor, Thielavin B vs. control (1% vol/vol DMSO); (C) 1 µM adrenaline vs. no-addition control. Results are the percent [³H]-counts released from the cells after 30- or 15-min chase periods relative to cell-associated [³H]-counts in no-chase cells. (A) n = 8; (B) n = 6; and (C) n = 7 independent experiments, indicated p-values calculated with Student’s paired t test, two-tailed.