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Construction of shuttle vectors for expression of PA in B. anthracis. Plasmid pBLKSPPA is a pBluescript vector which contains the anthrax PA gene and its endogenous promoter inserted into a multiple cloning site on a ClaI-BamHI fragment. This vector was digested with BamHI, treated with phosphatase, and ligated to gram-positive vectors pUB110 and pC194, which had been digested with BamHI and BstYI, respectively, to create PA-producing shuttle vectors pJB1, pJB2, and pJB3.
