TABLE 2.
Plasmid stability in the absence of antibiotic selection in vitro
| Strain (plasmid) | % Kanr or Cmr
|
No. of generations | % Plasmid loss/ generation | |
|---|---|---|---|---|
| Initial | Final | |||
| ΔANR(pPA102) | 95.2 ± 1.1a | 113 ± 9.7 | 17.3 ± 0.1 | NLOb |
| ΔSterne(pPA102) | 104.3 ± 4.7 | 92.4 ± 5.2 | 17.8 ± 0.5 | 0.8 ± 0.2 |
| ΔANR(pJB1) | 80.6 ± 4.7 | 50.7 ± 15.6 | 17.3 ± 0.6 | 3.2 ± 1.4 |
| ΔSterne(pJB2) | 91.1 ± 4.3 | 1.7 ± 0.4 | 19.7 ± 0.4 | 18.6 ± 1.1 |
| ΔANR(pJB3) | 96.4 ± 7.8 | 0.7 ± 0.4 | 18.2 ± 0.5 | 25.0 ± 2.5 |
| ΔSterne(pJB3) | 91.9 ± 4.2 | 77.7 ± 8.0 | 18.3 ± 0.3 | 1.2 ± 0.8 |
a
Results all represent the mean ± the standard error of the mean of three determinations.
b
NLO, no loss observed in three independent cultures.