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. 2022 Nov 4;130(11):117003. doi: 10.1289/EHP11360

Figure 2.

Figures 2A to 2H are eight bar graphs, plotting Cer (Normalized L C-M S Area), ranging from 0.0 to 1.5 in increments of 0.5; S M (Normalized L C-M S Area), ranging from 0 to 8 in increments of 2; L P C (Normalized L C-M S Area), ranging from 0 to 20 in increments of 5; L P E (Normalized L C-M S Area), ranging from 0.0 to 2.0 in increments of 0.5; P C (Normalized L C-M S Area), ranging from 0 to 30 in increments of 10; P E (Normalized L C-M S Area), ranging from 0 to 800 in increments of 200; cholesterol ester (Normalized L C-M S Area), ranging from 0.0 to 0.4 in increments of 0.1; and T A G (Normalized L C-M S Area), ranging from 0 to 800 in increments of 200 (y-axis) across uppercase c plus vehicle, uppercase c plus P F O S, uppercase i plus vehicle, uppercase i plus P F O S, uppercase p plus vehicle, and uppercase p plus P F O S (x-axis).

Liver lipid profiles in mice exposed to PFOS and fed with diets supplemented with different fibers (C: cellulose as control, I: inulin, P: pectin). Lipids including sphingolipids (Cer, A; SM, B), lysophospholipids (LPC, C; LPE, D), phospholipids (PC, E; PE, F), cholesterol ester (ChE, G), and a neutral lipid (TAG, H) were analyzed using UHPLC-Q exactive MS. The normalized peak areas of lipid species in each lipid class are summarized. Bars represent means±SEMs of 6–8 mice in each group. Data were compared using two-way ANOVA and Tukey’s post hoc test for multiple comparisons, *p<0.05; **p<0.01; ***p<0.001; and ****p<0.0001. Detailed lipidomic data are listed in Excel Table S12. Note: ANOVA, analysis of variance; Cer, ceramide; LC-MS, liquid chromatography-mass spectrometry; LPC, lysophosphatidylcholine; LPE, lysophosphatidylethanolamine; PC, phosphatidylcholine; PE, phosphatidylethanolamine; PFOS, perfluorooctane sulfonate; SEM, standard error of the mean; SM, sphingomyelin; UHPLC-Q exactive MS, ultra-high-performance liquid chromatography coupled with quadrupole-exactive mass spectrometer; TAG, triacylglycerol.