Table 2.
Sequences of primers used in this study.
| Null mutant | Primer name | Primer sequence | Reference |
|---|---|---|---|
| V.cholerae ΔcqsA | cqsA_UF | GAGCTCGATATCGCATGCTGCCCCCTTCACAAGC | This study |
| cqsA_UR | CACCGTAGTTGACCGCATCATCAGGAAGTTGAGGCTTG | ||
| cqsA_DF | CAAGCCTCAACTTCCTGATGATGCGGTCAACTACGGTG | ||
| cqsA_DR | CAAGCTTCTTCTAGAGGTACCCGCAGGGAGAACTACTGC | ||
| Gene | Primer name | Primer sequence | Reference |
| Rpoa | rpoA_qPCR_F rpoA_qPCR_R |
GGCGCTCATCTTCTTCCGAAT CGCGGTCGTGGTTATGTG |
64 |
| espB | EspB_qPCR_F EspB_qPCR_R |
GGCTCTTTTGCTGCCATTAATAGC TCTGCTGCATCTGCAATACC |
7 |
| espA | EspA_qPCR_F EspA_qPCR_R |
GTGCGAATGCGAGTACTTCGAC TTGCAGCCTGAAAAACACCGAGT |
7 |
| Tir | Tir_qPCR_F Tir_qPCR_R |
GGACCCTCTGCATTTCGTGTTG GTCCCCCGGTAAAAACAAATCTG |
7 |