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. 2022 Nov 2;11(1):2141011. doi: 10.1080/2162402X.2022.2141011

Figure 3.

Figure 3.

Tbc1d10c is an intrinsic suppressor of CD8 T-cell activation and cytotoxic function. (A-C) Bar graphs showing the average percentages of CD69+ (A), CD25+ (B) and Ifn-γ+ (C) CD8 T cells from OT-I;Tbc1d10c+/+ and OT-I;Tbc1d10c−/− mice either unstimulated (U) or stimulated (O) with OVA peptide for the indicated times (n = 3 replicates per group). (D) Bar graphs showing flow cytometric detection of IFN-γ+ primary human CD8 T cells transfected with non-targeting (Scramble) or TBC1D10C-targeting siRNA pools (n = 3 replicates per group). (E) Bar graphs showing proliferation as measured by CFSE dilution in CD8 T cells from OT-I;Tbc1d10c+/+ and OT-I;Tbc1d10c−/− mice either unstimulated (U) or stimulated (O) with OVA peptide for the indicated times (n = 3 replicates per group). (F) Bar graphs showing the average migration index of CD8 T cells from Tbc1d10c+/+ and Tbc1d10c−/− mice in the presence of 1, 10, and 100 ng/ml Cxcl10 (n = 3 replicates per group). (G) Line graphs showing average MC38 cell lysis by OT-I;Tbc1d10c+/+and OT-I;Tbc1d10c−/− CD8 T cells (n = 3 replicates per ratio group). (H-I) Line graph showing average B16-OVA tumor size in Rag2−/− mice adoptively transferred with OT-I CD4 and CD8 T cells (H; n = 5 mice per group) or OT-I CD8 T cells alone (I; n = 9 mice per group) carrying the indicated (WT or KO) Tbc1d10c genotype. P values were determined by unpaired Student’s t-test. Abbreviation: n.s., not significant.