Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2022 Nov 2;11(1):2141011. doi: 10.1080/2162402X.2022.2141011

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2022 The Author(s). Published with license by Taylor & Francis Group, LLC.

This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial License (http://creativecommons.org/licenses/by-nc/4.0/), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

PMC Copyright notice

Figure 6. — Assessment of IκBα and Map3k3 expression in Tbc1d10c^−/− CD8 T cells. (A-B) Gel images (A) and densitometric quantification (B) of total and phosphorylated p65 and IKKβ protein levels in Tbc1d10c^+/+and Tbc1d10c^−/− CD8 T cells following CD3/CD28 activation at the indicated time points (n = 2 replicates per group). (C-D) Representative dot plots of flow cytometric detection of p-IKK levels of SCC-infiltrating CD3+ CD8 + T cells and bar graph (D) showing average percentage of p-IKK+ CD8 T cells (n = 4 tumors per group). Histogram shows an overlay of representative p-IKK+ cell numbers in Tbc1d10c^+/+and Tbc1d10c^−/− cohorts. (E) Representative dot plots of flow cytometric detection of p-IKK+ human CD8 T cells subjected to TBC1D10C siRNA or control (si-Scramble) silencing and vehicle (unstimulated) or CD3/CD28 stimulation. (F) Bar graph showing the average percentage of p-IKK+ human CD8 T cells subjected to TBC1D10C siRNA or control (si-Scramble) silencing and CD3/CD28 stimulation (n = 5 replicates per group). (G-I) Gel images and densitometric quantification (bar graphs) showing relative total and phosphorylated protein levels of indicated proteins in Tbc1d10c^+/+and Tbc1d10c^−/− CD8 T cells (n = 3 replicates per group). (J) Bar graphs showing relative mRNA expression of Bcl10 and Map3k3 as determined by QPCR (n = 3 per replicates per group). (K) Bar graphs showing relative total and phosphorylated TAK1 protein levels in Tbc1d10c^+/+and Tbc1d10c^−/− CD8 T cells from panel G (n = 3 replicates per group). P values were determined by unpaired Student’s t-test.