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Expression of IFN-γ mRNA after immunization with L. monocytogenes ATCC 15313 or EGD as determined by RT-PCR. Mice were immunized with 2 × 107 CFU of ATCC 15313 (lane b), ATCC 15313 plus 200 μl of LLO-liposome (lane c), or 2 × 103 CFU of EGD (lane d). Lane a, control; lane M, 100 bp ladder. Total cellular RNA was extracted from the spleen 24 h after stimulation for RT-PCR.
