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. 2022 Nov 4;8(44):eabo2343. doi: 10.1126/sciadv.abo2343

Table 1. Table of DNA constructs.

L, linker.

Constructs Reference Details
APC2-C-RFP-FRB (41)
CAAX-iLID Addgene (#85680)
FKBP-mCherry-CAAX This paper 2xFKBP-L-mCherry-
CAAX
K353-GCN4-GFP This paper hKif5b(1–353)-GCN4-
L-GFP
K353-GCN4-mCherry This paper hKif5b(1–353)-GCN4-
L-mCherry
K560-GFP This paper hKif5b(1–560)-L-GFP
K560Rigor-mNeon-FRB This paper hKif5b(1–560)
Rigor-
2xmNeongreen-FRB
K560Rigor-HA This paper hKif5b(1–560)
Rigor-L-2xHA
K560Rigor-HA-FRB This paper hKif5b(1–560)
Rigor-L-2xHA-L-FRB
K560Rigor-mVenus This paper hKif5b(1–560)
Rigor-L-mVenus
K560Rigor-mVenus-
SspBmicro
This paper hKif5b(1–560)
Rigor-L-mVenus-L-
SspB(micro)
K807-GFP-FRB (20)
K807-mCherry-FKBP This paper hKif5b(1–807)-L-
mCherry-L-FKBP
LifeAct-mCherry This paper
mRFP-Fill (42)
PA-tubulin (42) PA-GFP tubulin
PA-Rac1 Addgene (#22024)
TRIM46-GFP (17)
TRIM46-mCherry (17)