Table 2. Table of chemicals.
Chemicals | Source | Reference |
16% Paraformaldehyde EM grade |
Electron Microscopy Sciences |
Catalog #15710 |
2% Bis-acrylamide solution | Fisher Scientific | Catalog #BP1404 |
3-(Trimethoxysilyl)propyl methacrylate |
Sigma-Aldrich | Catalog #M6514 |
40% Acrylamide solution | Sigma-Aldrich | Catalog #A4058 |
8% Glutaraldehyde solution | Sigma-Aldrich | Catalog #G7526 |
Acetic acid (J. T. Baker) | Fisher Scientific | Catalog #15578364 |
Alexa Fluor Plus 405 phalloidin |
Thermo Fisher Scientific |
Catalog #A30104 |
Ammonium persulfate | Sigma-Aldrich | Catalog #215589 |
B27 supplement | Gibco | Catalog #17504001 |
Bovine serum albumin (BSA) |
Carl Roth | Catalog #8076.3 |
DMSO, anhydrous | Thermo Fisher Scientific |
Catalog #D12345 |
EHT1864 (Rac inhibitor) | Tocris | Catalog #872 |
Ethanol | Fisher Scientific | Catalog #15568474 |
FluoSpheres carboxylate- modified microspheres, 0.2 μm, dark red fluorescent (660/680), 2% solids |
Thermo Fisher Scientific |
Catalog #F8807 |
FuGENE 6 | Promega | Catalog #E2692 |
l-Glutamic acid | Sigma-Aldrich | Catalog #G1251 |
l-Glutamine | Gibco | Catalog #25030-081 |
Hydrazine hydrate | Sigma-Aldrich/ Merck |
Catalog #225819 |
Laminin | Roche | Catalog #1124321700 |
Jasplakinolide | Tocris/ Bio-Techne |
Catalog #2792 |
Leibovitz’s L-15 medium | Thermo Fisher Scientific |
Catalog #11415064 |
Lipofectamine 2000 | Thermo Fisher Scientific |
Catalog #11668019 |
ML141 (Cdc42 inhibitor) | Tocris | Catalog #4266 |
Neurobasal medium | Gibco | Catalog #21103049 |
PBS | Lonza | Catalog #BE17-517Q |
Penicillin/streptomycin | Gibco | Catalog #15140122 |
Poly-l-lysine hydrobromide | Sigma-Aldrich | Catalog #P2636 |
Prolong Diamond antifade mountant |
Thermo Fisher Scientific |
Catalog #P3670 |
Rapalog | Takara | Catalog #635057 |
TEMED | Bio-Rad | Catalog #610801 |