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. 2022 Nov 4;8(44):eabo2343. doi: 10.1126/sciadv.abo2343

Table 2. Table of chemicals.

DMSO, dimethyl sulfoxide; PBS, phosphate-buffered saline; TEMED, N,N,N′,N′-tetramethylethylenediamine.

Chemicals Source Reference
16% Paraformaldehyde EM
grade
Electron
Microscopy
Sciences
Catalog #15710
2% Bis-acrylamide solution Fisher Scientific Catalog #BP1404
3-(Trimethoxysilyl)propyl
methacrylate
Sigma-Aldrich Catalog #M6514
40% Acrylamide solution Sigma-Aldrich Catalog #A4058
8% Glutaraldehyde solution Sigma-Aldrich Catalog #G7526
Acetic acid (J. T. Baker) Fisher Scientific Catalog #15578364
Alexa Fluor Plus 405
phalloidin
Thermo Fisher
Scientific
Catalog #A30104
Ammonium persulfate Sigma-Aldrich Catalog #215589
B27 supplement Gibco Catalog #17504001
Bovine serum
albumin (BSA)
Carl Roth Catalog #8076.3
DMSO, anhydrous Thermo Fisher
Scientific
Catalog #D12345
EHT1864 (Rac inhibitor) Tocris Catalog #872
Ethanol Fisher Scientific Catalog #15568474
FluoSpheres carboxylate-
modified microspheres,
0.2 μm, dark red
fluorescent (660/680),
2% solids
Thermo Fisher
Scientific
Catalog #F8807
FuGENE 6 Promega Catalog #E2692
l-Glutamic acid Sigma-Aldrich Catalog #G1251
l-Glutamine Gibco Catalog #25030-081
Hydrazine hydrate Sigma-Aldrich/
Merck
Catalog #225819
Laminin Roche Catalog #1124321700
Jasplakinolide Tocris/
Bio-Techne
Catalog #2792
Leibovitz’s L-15 medium Thermo Fisher
Scientific
Catalog #11415064
Lipofectamine 2000 Thermo Fisher
Scientific
Catalog #11668019
ML141 (Cdc42 inhibitor) Tocris Catalog #4266
Neurobasal medium Gibco Catalog #21103049
PBS Lonza Catalog #BE17-517Q
Penicillin/streptomycin Gibco Catalog #15140122
Poly-l-lysine hydrobromide Sigma-Aldrich Catalog #P2636
Prolong Diamond
antifade mountant
Thermo Fisher
Scientific
Catalog #P3670
Rapalog Takara Catalog #635057
TEMED Bio-Rad Catalog #610801