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. 2022 Oct 6;11:e80604. doi: 10.7554/eLife.80604

Figure 5. Frp1 and Pga7 collaborate in heme uptake.

Figure 5.

(A) Saccharomyces cerevisiae hem1Δ cells (KY1498) were transformed with a vector plasmid, or with plasmids HTB2p-FRP1 (KB2569), HTA2p-PGA7(KB2789) or HTB2p-FRP1 HTA2p-PGA7 (KB2566) and drop-diluted on SC-HIS plates supplemented with either 0.2 μM hemin or 50 μg/ml δ-aminolevulinic acid (ALA), as indicated. The plates were incubated for 2 days at 30°C. (B) The same strains were diluted in SC-HIS medium supplemented with the indicated amounts of hemin, and incubated at 30°C for 2 or 3 days, as indicated. For each plasmid, three independent transformant colonies were grown. The data indicate the average of the three cultures, and the error bars indicate the standard deviations. Statistically significant differences compared to vector control are indicated with one asterisk (p<0.05) or two asterisks (p<0.001) (Student’s t-test).

Figure 5—source data 1. Excel file with data used to make Figure 5B.