Key resources table.
| Reagent type (species) or resource | Designation | Source or reference | Identifiers | Additional information |
|---|---|---|---|---|
| Strain, strain background (Candida albicans) | WT | Fonzi and Irwin, 1993 | KC2=CAF3-1 | ura3Δ::imm434/ura3Δ::imm434 |
| Strain, strain background (Candida albicans) | ccc2-/- | Weissman et al., 2002 | KC68 | KC2 ccc2Δ::hisG/ccc2Δ::hisG |
| Strain, strain background (Candida. albicans) | rbt5-/- | Weissman and Kornitzer, 2004 | KC139 | KC68 rbt5Δ/rbt5Δ |
| Strain, strain background (Candida. albicans) | pga7-/- | Kuznets et al., 2014 | KC485 | KC68 pga7Δ/pga7Δ |
| Strain, strain background (Candida albicans) | WT | Fonzi and Irwin, 1993 | KC590=CAI4 | ura3Δ::imm434/ura3Δ::imm434 |
| Strain, strain background (Candida albicans) | pga7-/- | Kuznets et al., 2014 | KC646 | KC590 pga7Δ/pga7Δ ADE2/ade2::URA3 |
| Strain, strain background (Candida. albicans) | pga7-/- PGA7 URA3 | Kuznets et al., 2014 | KC647 | KC590 pga7Δ/pga7Δ ADE2/ade2::PGA7 URA3 |
| Strain, strain background (Candida albicans) | pga7-/- URA3 | This work | KC811 | KC68 ADE2/ade2::URA3 |
| Strain, strain background (Candida albicans) | FRP1+/- | This work | KC859 | KC2 FRP1/frp1Δ |
| Strain, strain background (Candida albicans) | frp1-/- | This work | KC870 | KC2 frp1Δ/frp1Δ |
| Strain, strain background (Candida albicans) | FRP1+/- | This work | KC901 | KC2 FRP2/frp2Δ |
| Strain, strain background (Candida albicans) | frp2-/- | This work | KC912 | KC2 frp2Δ/frp2Δ |
| Strain, strain background (Candida albicans) | frp2-/- | This work | KC913 | KC68 frp2Δ/frp2Δ |
| Strain, strain background (Candida albicans) | FRP1-GFP | This work | KC914 | KC2 FRP1/FRP1-GFP URA3 |
| Strain, strain background (Candida albicans) | FRP1-GFP | This work | KC916 | KC2 frp1Δ/FRP1-GFP URA3 |
| Strain, strain background (Candida albicans) | frp1-/- | This work | KC923 | KC68 frp1Δ/frp1Δ |
| Strain, strain background (Candida albicans) | frp1-/- | This work | KC966 | KC590 frp1Δ/frp1Δ |
| Strain, strain background (Candida albicans) | frp1-/- URA3 | This work | KC1023 | KC870 ADE2/ade2::URA3 |
| Strain, strain background (Candida albicans) | frp1-/- FRP1 URA3 | This work | KC1024 | KC870 ADE2/ade2::FRP1 URA3 |
| Strain, strain background (Candida albicans) | frp2-/- | This work | KC1053 | KC590 frp2Δ/frp2Δ |
| Strain, strain background (Candida albicans) | frp1-/- frp2-/- | This work | KC1061 | KC590 frp1Δ/frp1D frp2Δ/frp2Δ |
| Strain, strain background (Candida albicans) | frp1-/- FRP1p-FRP2 | This work | KC1064 | KC870 ADE2/ade2::FRP1p-FRP2 URA3 |
| Strain, strain background (Candida albicans) | SSB1p-FRP1 | This work | KC1080 | KC870 ADE2/ade2::SSB1p-FRP1 URA3 |
| Strain, strain background (Candida albicans) | frp1-/- URA3 | This work | KC1146 | KC923 ADE2/ade2::URA3 |
| Strain, strain background (Candida albicans) | SSB1p-FRP2 | This work | KC1244 | KC912 ADE2/ade2::SSB1p-FRP2 URA3 |
| Strain, strain background (Candida albicans) | FRP2-GFP | This work | KC1245 | KC912 frp2Δ/FRP2-GFP URA3 |
| Strain, strain background (Candida albicans) | frp2-/- URA3 | This work | KC1246 | KC912 ADE2/ade2::URA3 |
| Strain, strain background (Candida albicans) | frp2-/- FRP2 URA3 | This work | KC1379 | KC912 ADE2/ade2::FRP2 URA3 |
| Strain, strain background (Candida albicans) | FRP2-GFP | This work | KC1405 | KC2 FRP2/FRP2-GFP URA3 |
| Strain, strain background (Candida albicans) | frp1-/- frp2-/- | This work | KC1410 | KC2 frp1Δ/frp1D frp2Δ/frp2Δ |
| Strain, strain background (Candida albicans) | frp2-/- FRP2 URA3 | This work | KC1411 | KC913 ADE2/ade2::FRP2 URA3 |
| Strain, strain background (Candida albicans) | frp2-/- URA3 | This work | KC1412 | KC923 frp2Δ/frp2Δ::hisG-URA3-hisG |
| Strain, strain background (Candida albicans) | frp2-/- URA3 | This work | KC1414 | KC913 ADE2/ade2::URA3 |
| Strain, strain background (Candida albicans) | frp2-/- FRP1p-FRP2 | This work | KC1447 | KC912 ADE2/ade2:: FRP1p-FRP2 URA3 |
| Strain, strain background (Sandida cerevisiae) | hem1 | This work | KY1498 | ura3-1 can1-100 GAL+leu2-3,112 trp1-1 ade2-1 his3-11,15 hem1Δ::KanMX |
| Antibody | Anti-GFP (Rabbit polyclonal) | Abcam | Cat # ab290 | Use at 1:5000 |
| Antibody | Anti-rabbit IgG, HRP-conjugated (Goat polyclonal) | Sigma | Cat # A9169 | Use at 1:5000 |
| Recombinant DNA reagent | FRP1 blaster plasmids | This work | KB2392 KC2393 |
Digest SacI-KpnI for transformation |
| Recombinant DNA reagent | FRP2 blaster plasmids | This work | KB2395 KB2396 |
Digest SacI-KpnI for transformation |
| Recombinant DNA reagent | FRP1 reintegrant | This work | KB2546 | Contains the FRP1 region from –395 to +1877 |
| Recombinant DNA reagent | FRP2 reintegrant | This work | KB2576 | Contains the FRP2 region from –895 to +1982 |
| Recombinant DNA reagent | FRP1-GFP | This work | KB2431 | Contains FRP1 (+3 to+1662) fused to eGFP |
| Recombinant DNA reagent | FRP2-GFP | This work | KB2695 | Contains FRP2 (–969 to +1776) fused to eGFP |
| Recombinant DNA reagent | FRP1p-FRP2 | This work | KB2575 | Contains the FRP1 promoter (−395 to –1) fused to the FRP2 (+1 to +1878) |
| Recombinant DNA reagent | SSB1p-FRP1 | This work | KB2450 | Contains FRP1 (–2 to +1826) under SSB1 promoter (−400 to –1) |
| Recombinant DNA reagent | SSB1p-FRP2 | This work | KB2696 | Contains FRP2 (–3 to +1933) under SSB1 promoter (−400 to –1) |
| Recombinant DNA reagent | PGA7 FRP1 | This work | KB2566 | H2Ap-PGA7, H2Bp-FRP1 codon corrected |
| Recombinant DNA reagent | FRP1 | This work | KB2569 | H2Bp-FRP1 codon corrected |
| Recombinant DNA reagent | PGA7 | This work | KB2789 | H2Ap-PGA7 |
| Sequence-based reagent | FRP1 codon-corrected | This work | B35984-1/M131416 | FRP1 codon-corrected in pUC57 |
| Sequence-based reagent | SacI-FRP1 5’ (–795) | This work | PCR primer 1503 | GCGAGCTCCCAGCAGCACTTCCTG |
| Sequence-based reagent | FRP1 5’ (–1) – SpeI | This work | PCR primer 1504 | ggactaGTTGAAAGTTAAACTTGGTTA |
| Sequence-based reagent | HindIII-FRP1 3’ (+1662) | This work | PCR primer 1505 |
GGGAAGCTTAGGGTATATAGGATAAAT |
| Sequence-based reagent | FRP1 3’ (+2258) - KpnI | This work | PCR primer 1506 | gcggtACCCAAATGCATGGGTAAAC |
| Sequence-based reagent | FRP1 test (–810) | This work | PCR primer 1507 | CACTTGCACTACCAGTTTCG |
| Sequence-based reagent | SacI-FRP2 5’ (–730) | This work | PCR primer 1508 | GGGAGCTCGGAAAATAAGTTGTTCTTTG |
| Sequence-based reagent | FRP2 5’ (+3) – SpeI | This work | PCR primer 1509 | CGACTAGTCCATGGCTGATAAGTTG |
| Sequence-based reagent | HindIII-FRP2 3’ (+1753) | This work | PCR primer 1510 | GGGAAGCTTCTATAACGAGTCGTACGA |
| Sequence-based reagent | FRP2 3’ (+2394) – KpnI | This work | PCR primer 1511 | CCGGTACCTGATCCTTGGATGCCA |
| Sequence-based reagent | FRP2 test (–750) | This work | PCR primer 1512 | GTAACAAACCCGAGAACACC |
| Sequence-based reagent | RI-FRP1 | This work | PCR primer 1513 | CCGAATTCAACCATGGCTATTCCAT |
| Sequence-based reagent | FRP1(+1740)-XhoI | This work | PCR primer 1514 | cgctcgaGGTGTGTCCTTACGTACAG |
| Sequence-based reagent | RI-FRP2 | This work | PCR primer 1515 | gcGAATTCCATGGACGAAGAACTTCAG |
| Sequence-based reagent | FRP2 (+1872)-XhoI | This work | PCR primer 1516 | ggctcGAGAGTGCTGTGAGGTTATG |
| Sequence-based reagent | BamHI-FRP1(+3) | This work | PCR primer 1522 | gcggatccgctattccatttgatcaacag |
| Sequence-based reagent | FRP1(+1662)-XhoI | This work | PCR primer 1523 | cgctcgagaaacgactctgtataacaatac |
| Sequence-based reagent | SpeI-FRP1 5' (–395) | This work | PCR primer 1541 | ccACTAGTCGTAATCAGCAGCAGATAC |
| Sequence-based reagent | FRP1 3' (+1877) - KpnI | This work | PCR primer 1542 | ccGGTACCGCACAAGCGGGTACT |
| Sequence-based reagent | FRP1 (+1550)-XhoI | This work | PCR primer 1543 | GGCTCGAGTTTGTGAATGATGGCGA |
| Sequence-based reagent | PGA7 (+1087)-HindIII | This work | PCR primer 1544 | GCAAGCTTGGCATACTCAATTTGATG |
| Sequence-based reagent | 5’-F1HEM1 | This work | PCR primer | CCCTCAATAATCATAACAGTACTTAGGTTTTTTTTTCAGTCGGATCCCCGGGTTAATTAA |
| Sequence-based reagent | 3’-R1HEM1 | This work | PCR primer | CCTTGTACCTCTATCTCAGCCCATGCATATATTGGTTGTTGAATTCGAGCTCGTTTAAAC |
| Sequence-based reagent | Promoter 5’ (HTA2) | This work | PCR primer | TATATATTAAATTTGCTCTTGTTC |
| Sequence-based reagent | Promoter 3’ (HTB2) | This work | PCR primer | TAGTTGTAGAGTAAGTTGTTG |
| Sequence-based reagent | SacI-HTB2p –684 | This work | PCR primer | gcgaGCTCTTGTTCTGTACTTTCC |
| Sequence-based reagent | PGA7 5’ | This work | PCR primer | GAACAAGAGCAAATTTAATATATAATGCATTTCATATTCTACTTGA |
| Sequence-based reagent | Pga7-SacI (+697) | This work | PCR primer | Pga7-SacI (+697) |
| Sequence-based reagent | FRP1 5’ | This work | PCR primer | CAACAACTTACTCTACAACTAATGGCTATTCCATTTGATCAA |
| Sequence-based reagent | FRP1-SalI | This work | PCR primer | Ccccgtcgacggtatcga |
| Chemical compound, drug | Hemin | Frontier Scientific | H651-9 | Hemin chloride |
| Chemical compound, drug | Bovine hemoglobin | Sigma-Aldrich | H2500 | |
| Chemical compound, drug | Ferrozine | Sigma-Aldrich | P9762 | 3-(2-Pyridyl)–5,6-diphenyl-1,2,4-triazine-4’,4”-disulfonic acid sodium salt |
| Chemical compound, drug | BPS | Sigma-Aldrich | B1375 | Bathophenanthroline sulfonate |
| Chemical compound, drug | ALA | Merck | 08339 | δ-Aminolevulinic acid |
| Chemical compound, drug | GaPPIX | Frontier Scientific | P40167 | Ga3+-protoporphyrin IX chloride |
| Chemical compound, drug | CoPPIX | Frontier Scientific | Co654-9 | Co3+-protoporphyrin IX chloride |
| Chemical compound, drug | MnPPIX | Frontier Scientific | MnP562-9 | Mn3+-protoporphyrin IX chloride |
| Chemical compound, drug | ZnPPIX | Frontier Scientific | Zn625-9 | Zn2+-protoporphyrin IX |
| Chemical compound, drug | ZnMP | Chem-Cruz | Sc-396862 | Zn2+-mesoporphyrin |
| Other | Alignment summary of 40 saccharomycetales species for phylogenetic profiling | This work | github.com/BKU-Technion/FRP |