Table 2. Reagents and resources.
| Reagent or resource | Source | Identifier |
|---|---|---|
| Mouse strains | ||
| C57BL/6J (wild-type) | JAX | 000664 |
| B6.FVB(Cg)-Tg(Th-cre)FI172Gsat/Mmucd | MMRRC | 031029-UCD |
| B6;129S-Gt(ROSA)26Sortm38(CAG-GCaMP3)Hze/J | JAX | 014538 |
| B6.Higd1c 3-1 | This paper | |
| B6.Higd1c 1-1 | This paper | |
| B6.Higd1c 5-3 | This paper | |
| sgRNA primers (target sequence in bold) | ||
| sgRNA-1 (forward): TAATACGACTCACTATAGGGAGTCTCTCGATTTCCGGGTTTTAGAGCTAGAA | This paper | |
| sgRNA-2 (forward): TAATACGACTCACTATAGGCTGATTTAAGGAGTGAGTGCGTTTTAGAGCTAGAA | This paper | |
| sgRNA (reverse, common): AAAAAAAGCACCGACTCGGTGCCACTTTTTCAAGTTGATAACGGACTA GCCTTATTTTAACTTGCTATTTCTAGCTCTAAAAC |
This paper | |
| Genotyping primers | ||
| Higd1c-P8: GTCAGGTGGCCCCTGATGAAA | This paper | |
| Higd1c-P9: GTGCACGAGCAGACTGGTTCT | This paper | |
| Higd1c-P11: GGATATCACAGCCACAGAGGACG | This paper | |
| Mouse qPCR primers | ||
| Actb-F: AGCCATGTACGTAGCCATCC | This paper | |
| Actb-R: GCCATCTCTTGCTCGAAGTC | This paper | |
| Higd1c (5 exon)-F: CACGTACAAGGGCTGCATGG | This paper | |
| Higd1c (5 exon)-R: ACCTAGAGTCACGGCTCCC | This paper | |
| Higd1c (4 exon)-F: CCAGCACGTACAAGAGAGAAA | This paper | |
| Higd1c (4 exon)-R: ACGTGGATGAGATGAAGGGAC | This paper | |
| Rat qPCR primers | ||
| GADPH-F: CAAGTTCAACGGCACAGTCAAG | Kim et al., 2011 | |
| GADPH-R: ACATACTCAGCACCAGCATCAC | Kim et al., 2011 | |
| Higd1c-F1: CCTGTGCTGATCAAAGAGCA | This paper | |
| Higd1c-R1: CTGACCACTCATCTGAAGAC | This paper | |
| Higd1c-R2: CTGCTGACCACTCATCTGAA | This paper | |
| Kcnk3-F: GCAGAAGCCGCAGGAGTTC | Kim et al., 2011 | |
| Kcnk3-R: GCCCGCACAGTTGGAGATTTAG | Kim et al., 2011 | |
| Kcnk9-F: CGGTGCCTTCCTCAATCTTGTG | Kim et al., 2011 | |
| Kcnk9-R: TGGTGCCTCTTGCGACTCTG | Kim et al., 2011 | |
| Th-F: TCGGAAGCTGATTGCAGAGA | Feng et al., 2020 | |
| Th-R: TTCCGCTGTGTATTCCACATG | Feng et al., 2020 | |
| Olr59-F: TCATTCACGCTCTCTCAGCA | von der Weid et al., 2015 | |
| Olr59-R: CCATGCCGATTTGGACTGTT | von der Weid et al., 2015 | |
| Human qPCR primers | ||
| GADPH-F: ACCACAGTCCATGCCATCAC | Maßberg et al., 2016 | |
| GADPH-R: TCCCACCACCCTGTTGCTGTA | Maßberg et al., 2016 | |
| HIGD1A-F1: CAACAGACACAGGTGTTTCC | This paper | |
| HIGD1A-R1: CAATTGCTGCAAAACCCGCT | This paper | |
| HIGD2A-F: GCCCCACTGTTTACAGGAAT | This paper | |
| HIGD2A-R: GCGCATCATGAGCTGAGAG | This paper | |
| HIGD1C-F: GAAGGCCAATTATCCCGACT | This paper | |
| HIGD1C-R: GCTTGTAAAGACCACAGGAC | This paper | |
| COX4I1-F: CAAGCGAGCAATTTCCACCT | This paper | |
| COX4I1-R: CCTTCTCCTTCAATGCCTTC | This paper | |
| COX4I2-F: GAGGGATGCACAGCTCAGAA | This paper | |
| COX4I2-R: CTTCTCCTTCTCCTTCAGGG | This paper | |
| NDUFA4L2-F: GATGATCGGCTTAATCTGCC | This paper | |
| NDUFA4L2-R: GTATTGGTCATTGGGGCTCA | This paper | |
| TH-F: GCTGGACAAGTGTCATCACCTG | OriGene | HP234519 |
| TH-R: CCTGTACTGGAAGGCGATCTCA | OriGene | HP234519 |
| OR51E2-F2: TCATCCCATTGTGCGTGTTG | This paper | |
| OR51E2-R2: CACCCGTGTTCTGATCTGTTTG | This paper | |
| BaseScope in situ hybridization probes | ||
| BA-Mm-Higd1c-2zz-st | ACD | 862241 |
| BA-Mm-Th-3EJ-C2 | ACD | 854771-C2 |
| dapB-1ZZ-C1/dapB-1ZZ-C2 | ACD | 700141 |
| Mm-Ppib-1ZZ | ACD | 701081 |
| Mm-Polr2a-1ZZ-C2 | ACD | 701101-C2 |
| Plasmids | ||
| HIGD1C-Myc-FLAG in pCMV6-Entry (human) | OriGene | RC225015 |
| Higd1c-Myc-FLAG in pCMV6-Entry (mouse) | OriGene | MR220387 |
| COX4I1-Myc-FLAG in pCMV6-Entry | OriGene | RC209374 |
| COX4I2-Myc-FLAG in pCMV6-Entry | OriGene | RC209204 |
| pCMV6-A-Entry-Hygro | OriGene | PS100024 |
| pCMV6-A-Entry-BSD | OriGene | PS100022 |
| HIGD1A-Myc-FLAG in pCMV6-A-Entry-Hygro | Timón-Gómez et al., 2020b | |
| HIGD2A-Myc-FLAG in pCMV6-A-Entry-Hygro | Timón-Gómez et al., 2020b | |
| HIGD1C-Myc-FLAG in pCMV6-A-Entry-Hygro (human) | This paper | |
| Higd1c-Myc-FLAG in pCMV6-A-Entry-Hygro (mouse) | This paper | |
| COX4I1-Myc-FLAG in pCMV6-A-Entry-BSD | This paper | |
| COX4I2-Myc-FLAG in pCMV6-A-Entry-BSD | This paper | |
| Primary antibodies/stains | ||
| Mouse anti-DDK/FLAG | OriGene | TA50011 |
| Mouse anti-HSP60 | ECM Biosciences | HM-4381 |
| Rabbit anti-TH | Abcam | ab112 |
| Rat anti-CD31 | BD Pharmingen | 553370 |
| Lotus tetragonolobus lectin-Fluorescein | Vector Labs | FL-1321 |
| Mouse anti-ATP5A | Abcam | ab14748 |
| Mouse anti-β-ACTIN | Abcam | ab8227 |
| Mouse anti-CORE2 | Abcam | ab8227 |
| Mouse anti-COX1 | Abcam | ab14705 |
| Mouse anti-COX4I1 | Abcam | ab14744 |
| Rabbit anti-COX4I2 | Abnova | H00084701-M01 |
| Mouse anti-COX5B | Santa Cruz | sc-374417 |
| Mouse anti-β-TUBULIN | Sigma-Aldrich | C4585 |
| Rabbit anti-UQCRB | Abcam | ab122837 |
| Mouse anti-NDUFA9 | Abcam | ab14713 |
| Rabbit anti-NDUFB11 | Abcam | ab183716 |
| Rabbit anti-SDHA | Abcam | ab14715 |
| Mouse anti-cytochrome c | Santa Cruz | sc-13156 |