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. 2022 Oct 21;13:1025796. doi: 10.3389/fimmu.2022.1025796

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Copyright © 2022 Lee, Han, Lee, Lee, Park, Lee and Kim

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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The expression level of viperin, Mx, and OAS mRNA and protein expression in cultured normal and inflammatory sinonasal epithelial cells obtained from healthy control and patients with CRSwNP, respectively. Their levels were evaluated 24, 48, and 72 h after RV 16 inoculation and poly (I: C) treatment, which were evaluated by RT– qPCR (B, D) and western blot (A, C, E). Right panels located in the figure show representative protein bands evaluated with western blot. Data are mean ± SEM from 7 different epithelial donors. Normal ep + rhinovirus indicates that cultured epithelial cells were infected with RV. Inflam ep + rhinovirus indicates that cultured inflammatory epithelial cells were infected with RV. Normal ep + poly (I: C) means poly (I: C)-treated cultured normal epithelial cells. Inflam + poly (I:C) means poly (I:C)-treated cultured inflammatory epithelial cells. * indicates a significant difference between each time-points.