| Bacterial and virus strains |
|
|
|
| pAAV-hSyn-DIO-hM3D(Gq)-mCherry(AAV8) |
Addgene |
RRID: Addgene_44361 |
|
| Chemicals, peptides, and recombinant proteins |
|
|
|
| L-(−)-norepinephrine (+)-bitartrate salt monohydrate (NE) |
Sigma-Aldrich |
A9512, CAS 108341-18-0 |
| Picrotoxin |
Tocris |
Cat# 1128, CAS 124-87-8 |
| Corticosterone |
Tocris |
Cat# 3685, CAS 50-22-6 |
| Bobcat urine |
Maine Outdoor Solutions |
91412-1 |
| Lipopolysaccharides from Escherichia coli O26:B6 |
Sigma-Aldrich |
Cat# L2654 |
| 2-Methyl-1,2-di-3-pyridinyl-1-propa-none(metyrapone) |
Tocris |
Cat# 3292, CAS 54-36-4 |
| 3-hydroxynaphthalene-2-carboxylic acid (3,4-dihydroxybenzylidene) hydrazide (Dynasore) |
Tocris |
Cat# 2897, CAS 304448-55-3 |
| corticosterone:HBC |
Sigma-Aldrich |
C174, MDL MFCD00163376 |
| WGA Alexa Fluor 594 |
ThermoFisher |
W11262 |
|
| Critical commercial assays |
|
|
|
| Corticosterone RIA |
University of Virginia Center for Research in Reproduction Ligand Assay and Analysis Core |
RRID:SCR_004318 |
| corticosterone ELISA |
Enzo Life Sciences |
ADI-900-097 |
|
| Experimental models: Cell lines |
|
|
|
| Hypothalamic cell line mHypoE-N42 |
Cellutions Biosystems, Inc. |
RRID:CVCL_D443 |
|
| Experimental models: Organisms/strains |
|
|
|
| Mouse: Tg(CRH-EGFP)HS57Gsat/Mm |
MMRRC |
RRID:MMRRC_017058-UCD |
| Mouse: Sim1-cre |
Jackson Laboratory |
RRID:IMSR_JAX:006451 |
| Mouse: Loxp-GR |
(Laryea et al., 2013) |
N/A |
| Mouse: B6.Cg-7630403G23RikTg(Th-cre) 1Tmd/J |
Jackson Lab |
RRID:IMSR_JAX:008601 |
|
| Oligonucleotides |
|
|
|
| For genotyping primer sequences, please see Table S1
|
This paper |
N/A |
|
| Software and algorithms |
|
|
|
| Mini Analysis |
Synaptosoft, Inc. |
http://www.synaptosoft.com/MiniAnalysis/
|
| Graphpad Prism |
Graphpad |
https://www.graphpad.com/scientific-software/prism/
|
| SigmaPlot 11.0 |
Systat Software |
https://systatsoftware.com/products/sigmaplot/
|
