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. 2022 Nov 5;4:100125. doi: 10.1016/j.snr.2022.100125

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© 2022 The Authors. Published by Elsevier B.V.

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Fig. 4 — Sequence diagrams of stem alterations between generation 3 and 4 and their perfomance in a MG flourescecne assays. Sequence diagrams of the aptamer core region of SMG-3, SMG-4.1, and SMG-4.2 depicting alterations to the stem elements. To increase the stability of the upper and lower stem between SMG-3.1T and SMG-4.1 an A-T base pair in the upper stem was replaced with a G-C pair with extension of the lower stem by 2 base pairs. 4.2 destabilizes these stem elements by replacement of a pair of A-T with G-C base pairs in the central base pair of the stems. The resulting SBR calibration curve is shown right, with the SMG4.2T showing improved performance at all analyte concentrations. The above curve is the average of 3 independent trials and was processed on Microsoft Excel. Fluorescence was recorded at 658 nm with an excitation at 617 nm.