Fig. 14.

Fluorescence data for MG-1.0 derivatives with accompanying calibration curve Mini-MG 1.2 L. The fluorescence response at 658 nM of Mini-MG 1.0, 1.1 L and 1.2 L is shown on top. The inclusion of a hexaethylene glycol linker on the 3′ side of the active sequence simultaneously lowered background signal (dark gray) with an accompanying elevation of the fluorescence at 200 nM. Given the superior performance of Mini-MG 1.2 L, a calibration curve was created with a calculated LOD of 3.6 nM. The threshold signal, defined as the ${\overline{\mathit{x}}}_{\mathit{blank}}+3{\mathit{\sigma }}_{\mathit{Blank}}$, is indicated by the horizontal solid line. The above curve is the average of 3 independent trials and was processed on Microsoft Excel. Fluorescence was recorded at 658 nm with an excitation at 617 nm.