Fig. 15.

Calibration Curve and Discrimination Assay with Mini-MG 1.3. Top, the calibration curve of Mini-MG1.3 L is shown with a calculated LOD of 3.2 nM. The ability of Mini-MG 1.3 to discriminate between its target analyte NC-45 and mismatched analyte NC-45A and their corresponding long derivatives (NC-45 L and NC-45LA) is shown on the bottom. Mini-MG 1.3 was able to differentiate a G>A SNV with roughly a two-fold difference in signal which was further improved upon when the long-mismatched analyte, NC-45A, was used. The threshold signal, defined as the ${\overline{\mathit{x}}}_{\mathit{blank}}+3{\mathit{\sigma }}_{\mathit{Blank}}$, is indicated by the horizontal solid line. The above curve is the average of 3 independent trials and was processed on Microsoft Excel. Fluorescence was recorded at 658 nm with an excitation at 617 nm.