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. 2022 Oct 28;2022:1297888. doi: 10.1155/2022/1297888

Figure 6.

Figure 6

Induction of apoptosis in C. albicans ATCC10231 cells, where (a) Untreated C. albicans cells; (b) C. albicans cells treated with menthol (0.5 mM); and (c) C. albicans cells treated with menthol (1 mM). Menthol-treated apoptotic Candida albicans cells were stained with annexin V/PI and subjected to flow cytometry analysis. The four quadrants represent living cells R3 (annexin V-PI-), early apoptotic R4 (Annexin V + PI-), late apoptosis R2 (annexin + PI+) or necrotic or dead R1 (annexin V-PI+) stages. Quadrant analysis of fluorescence intensity of gated cells in BL3-H (annexin V-FITC) and BL1H (PI) channels was from 10,000 events. Values shown were percentages of each quadrant. ∗p < 0.05, in comparison to control.