Extended Data Fig. 8. M6A RT&Tag performance with decreasing number of nuclei input.
a) Heatmap showing the average IgG and m6A RT&Tag signal from two experiments performed using either 100,000 or 25,000 nuclei. Individual rows represent the 281 transcripts identified as m6A-enriched in Fig. 4b. The heatmap colors represent z-score scaling across rows. b) Genome browser tracks showing the distribution of IgG and m6A RT&Tag signal from 100,000, 25,000, or 5000 nuclei over the gene bodies of aqz and Syx1A. Combined reads from 2 replicates are shown.