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. 2022 Oct 24;19(11):1357–1366. doi: 10.1038/s41592-022-01621-0

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Extended Data Fig. 8 — a. Overview of 35 nm section of a 5 dpf larval zebrafish prepared identically to the sample used for SBEM. b. Close-up tile mosaic in optic tectum neuropil. Arrowhead points at synapse in (c). c. Example synapse. Dotted line illustrates synaptic contact length measurement. d. Distribution of contact lengths (n = 100, randomly sampled) in this TEM image (dotted line) compared to the distribution obtained from the optic tectum in the SBEM dataset (as in Extended Data Fig. 7b). Scale bars: a. 50 μm, b. 10 μm, c. 100 nm.