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. 2022 Oct 24;19(11):1357–1366. doi: 10.1038/s41592-022-01621-0

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 6 — a, Dorsal view of the vEM dataset, vesicle clouds labeled in blue. b, Close-up examples of automatically segmented vesicle clouds (blue) and synaptic clefts (magenta) in thalamus (Th), pretectum (preT), optic tectum (TeO) and ventral hindbrain (vHb). c. Outline of RGC AFs 4 (left) and 7 (right). d, Volume fraction occupied by vesicle clouds, calculated for all AFs as a percentage of total area volume. Visualization adapted from mapzebrain atlas. Scale bars, 50 µm (a), 500 nm (b) and 10 µm (c).