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. 2022 Oct 24;19(11):1357–1366. doi: 10.1038/s41592-022-01621-0

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Extended Data Fig. 3 — Individual example of a commissural pretectal interneuron (a) and all neurons of this type with somata on the right (b) and left (c) side of the brain. d-f. Like a-c, for ipsilateral descending pretectal projection neurons. g-i. Branchpoint densities (g), neurite path lengths (h) and axonal synapse densities (i) for commissural pretectal interneurons (blue, n = 18 and 7 cells on the left and right side, respectively) and ipsilateral descending pretectal projection neurons (red, n = 15 and 18 cells on the left and right side, respectively), plotted separately for axons and dendrites and for the two brain hemispheres. AF5, AF6: Retinal arborization fields 5 and 6, mPN: medial pretectal neuropil region, vHb: ventral hindbrain neuropil. AF annotations are based on LM atlas masks registered to the EM data. Box plot center lines represent medians, box limits upper and lower quartiles, whiskers 1.5x the interquartile range. Scale bar: 100 μm.