Skip to main content
. 2022 Oct 17;25(11):105375. doi: 10.1016/j.isci.2022.105375

Figure 5.

Figure 5

Inhibiting serotonin release by pretreating tadpoles with the tryptophan hydroxylase inhibitor pCPA weakens the preference for light

(A) Experimental time line: pCPA (5μM) is added to tadpoles rearing solution at 16:00 and tadpoles are returned to their incubator. Tadpoles are tested 24 h later, at 16:00 on the following day.

(B) Pre-treatment with pCPA decreases the average number of both stage 48 (left) and stage 49 (right) tadpoles residing in the light quadrant at any given time, suggesting that decreasing serotonin release decreases the preference for light. Each set of connected gray data points represents the average % of tadpoles in the light quadrant for batch-matched control and pCPA-treated group. The set of black connected data points represents the overall average of all the individual experiments (∗p < 0.05, ∗∗p < 0.01 as determined by Mann-Whitney U test).

(C) Plots showing the average number of stage 48 (top) and stage 49 (bottom) control and pCPA-treated tadpoles in the light quadrant at each minute of the 30 min trial. Error bars represent SEM.