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. 2022 Nov 4;12:18721. doi: 10.1038/s41598-022-22060-z

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Comparison of intra-host variability among several criteria between samples from persistent (yellow) and non-persistent (blue) patients. (a) global comparison of intra-host variability per sample. Welch’s t comparison test. (b) differences between clades. Kolmogorov–smirnov comparison test. (c) differences between severity of COVID-19. Unpaired t test used. (d) Hotspot mutations in spike domain. Mixed effect analysis, Šídák's multiple comparisons test. (e)Variance’s comparison in spike domain. Mixed effect analysis, Šídák's multiple comparisons test. (f) linear regression between intra-host variability and age. Simple linear regression test.