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. 2022 Nov 4;13:6562. doi: 10.1038/s41467-022-34306-5

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 5 — a, b Representative pseudo-color images of mitochondrial (a) or cytosolic (b) BioITA in RAW264.7 cells without or with Sting1 KO were captured at indicated time points post 2'3'-cGAMP (10 μg ml⁻¹) stimulation (a, b, left panels). Time-dependent fluorescence intensity of BioITAs or dBioITA in individual cell after 2'3'-cGAMP stimulation was quantitated (a, b, right panels). Mitochondrial or cytosolic dBioITA served as a control. Scale bar, 20 μm (a, b, left panels). Data are shown as mean ± SD (n = 8) (a, b, right panels). Dotted lines indicated the mean ± SD (a, b, right panels). Source data are provided as a Source Data file.