Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2022 Nov 4;13:6647. doi: 10.1038/s41467-022-34433-z

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Fig. 1 — a–c Human atheroma tissues with or without plaques were used to measure OASL expression. a OASL mRNA levels (p = 0.0463, n = 5) were measured by quantitative PCR. b Left: Immunofluorescence (IF) staining of OASL protein and CD31⁺ ECs (p < 0.0001, n = 5 per group). Right: Quantitation of mean fluorescence intensity (MFI). Scale bar, 50 μm. L, lumen. (c) Western blot analysis of OASL expression (n = 4 per group). d, e Atherosclerotic aortas were isolated from Apoe^−/− mice fed a normal chow diet (NCD) for 28 weeks. Oasl1 mRNA levels (p = 0.0313, n = 6) (d) and en face IF staining for OASL1 and CD31⁺ aortic ECs (n = 5 per group) (e) in matched aortic specimens with and without plaques are shown. Scale bar, 50 μm. f, g Aorta tissues were isolated from Apoe^+/+ and Apoe^−/− mice fed an NCD for 8 weeks. f mRNA levels of Oasl1 in aortas of each group (p = 0.0055, n = 6 per group) as measured by quantitative PCR. g Left: En face IF staining for OASL1 and CD31⁺ ECs in athero-prone or athero-resistant regions. Right: Quantitation of MFI (arch: p = 0.036, branch: p = 0.0008, descending: p = 0.9994, n = 4 per group). Scale bar, 50 μm. h, i OASL mRNA level (HUVEC: p = 0.017, HUAEC: p < 0.0001, n = 6 per group) (h) and immunocytochemical detection of OASL protein (HUVEC: p = 0.0222, HUAEC: p = 0.0017, n = 4 per group) (i) in HUVECs and HUAECs stimulated with TNFα and IFNγ. Scale bar, 20 μm. j, k Oasl1 mRNA level (p < 0.0001, n = 6 per group) (j) and immunocytochemical detection of OASL1 expression (p = 0.001, n = 10 per group) (k) in MAECs stimulated with TNFα and IFNγ. Scale bar, 20 μm. Data in b, e, g, i and k are representative of at least 3 independent experiments. Data are presented as the means ± SEMs (*p ≤ 0.05, **p ≤ 0.01, ***p ≤ 0.001, ****p ≤ 0.0001; a, two-sided paired t-test; d, two-sided Wilcoxon matched-pairs signed rank test; b, f, h–k, two-sided unpaired Student’s t-test; g, two-way ANOVA with Sidak’s test for multiple comparisons). Source data are provided as a Source Data file.