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. 2022 Nov 4;13:6647. doi: 10.1038/s41467-022-34433-z

Fig. 8. Endothelial OASL1 attenuates leukocyte recruitment and lesion formation.

Fig. 8

a–d Rescue effects of Oasl1 in vascular cell types were assessed by reconstituting γ-irradiated Oasl1−/−Apoe−/− and Apoe−/− mice with bone marrow (BM) from Oasl1−/−Apoe−/− mice followed by a normal chow diet (NCD) for 28 weeks (n = 8 for Oasl1−/−Apoe−/−; n = 9 for VcOasl1+/+-Apoe−/−). a Experimental scheme. b Left panel: Oil red O-stained lesions in whole aortas. Yellow arrowhead: plaque in aorta. Right panel: Quantification of stained en face area. Bottom panel: Separate quantitation of Oil red O+ areas in aortic arch (R1), descending (R2) and abdominal (R3) regions (Total: p = 0.0058, R1: p = 0.0203, R2: p = 0.0310, R3: p = 0.0159). c Blood flow velocity (top) and endothelial wall shear stress (WSS; bottom) at the athero-resistant greater curvature, descending and athero-prone lesser curvature, or abdominal branching point of arteries were assessed in age-matched 20-week-old mice prior to identification of plaque formation by serial echocardiography (velocity R1 (lesser): p = 0.0421, R3: p = 0.0383; WSS R1 (lesser): p = 0.0283, R3: p = 0.0478). d Flow cytometry analysis of single cells including total leukocytes, macrophages, neutrophils, and DCs, isolated from atherosclerotic aortas (Macrophage: p = 0.0499, Neutrophil: p = 0.0204, DC: p = 0.0427). e Assay of CFSE-labeled Oasl1−/− monocyte adhesion on TNFα- and IFNγ-stimulated MAECs isolated from Oasl1−/− and Oasl1+/+ mice (p = 0.0028, n = 10 per group). Scale bar, 50 μm. f Transendothelial migration assay of Oasl1−/− monocyte movement across TNFα- and IFNγ-stimulated Oasl1−/− and Oasl1+/+ MAECs (p = 0.0198, n = 10 per group). Scale bar, 100 μm. Data in b, e, and f are representative of each group. b–f, Box plots are shown as median of each value and the IQR; whiskers indicate 1.5 times the IQR. Data are presented as means ± SEMs (*p ≤ 0.05, **p ≤ 0.01, ***p ≤ 0.001; b–f, two-sided unpaired Student’s t-test). Source data are provided as a Source Data file.