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. 2022 Oct 14;54(10):1766–1777. doi: 10.1038/s12276-022-00850-9

Table 2.

The antibodies used in immunofluorescence staining (IF) and Western blot (WB).

Name Primary/secondary Application Manufacturer
Anti-Nestin antibody primary IF CST (Danvers, USA)
Anti-SOX2 antibody primary IF CST
Anti-MAP2 antibody primary IF and WB CST
Anti-β3-Tubulin antibody primary IF and WB CST
Anti-GFAP antibody primary IF and WB CST
Anti-NF-200 antibody primary IF and WB CST
Anti-NeuN antibody primary IF and WB CST
Anti-cleaved caspase-3 antibody primary IF and WB CST
Anti-neurofibromin antibody primary WB CST
Anti-P-mTOR antibody primary WB CST
Anti-Rictor antibody primary WB CST
Anti-GAPDH antibody primary WB CST
Anti-Bcl-2 antibody primary IF and WB CST
Anti-CNPase antibody primary IF and WB CST
Anti-myelin basic protein (MBP) antibody primary IF and WB CST
Alexa Fluor 594 goat anti-rabbit antibody secondary IF Invitrogen (Carlsbad, USA)
Alexa Fluor 594 goat anti-mouse antibody secondary IF Invitrogen
Goat anti-rabbit antibody secondary WB Invitrogen
Goat anti-mouse antibody secondary WB Invitrogen

CST Cell Signaling Technology, IF immunofluorescence staining, WB Western blot.