Table 1.
Published rodent malaria gene-deletion mutants with a ‘late-liver stage’ growth phenotype.
| berghei/yoelii gene ID | falciparum gene ID | product | blood infection | Extended prepatency | Ref. |
|---|---|---|---|---|---|
| PBANKA_1125100 | PF3D7_0626300 | 3-oxoacyl-acyl-carrier protein synthase I/II (FabB/FabF) | yes | yes | 70 |
| PY17X_1126500 | PF3D7_0626300 | 3-oxoacyl-acyl-carrier protein synthase I/II (FabB/FabF) | no | - | 71 |
| PBANKA_1229800 | PF3D7_0615100 | enoyl-acyl carrier reductase (ENR,FabI) | yes | yes | 72 |
| PY17X_1342900 | PF3D7_1323000 | beta-hydroxyacyl-ACP dehydratase (FabZ) | no | - | 71 |
| PBANKA_1410500 | PF3D7_1312000 | malonyl CoA-acyl carrier protein transacylase (FabD) | no | - | 23 |
| PBANKA_0308200 | PF3D7_0211400 | beta-ketoacyl-ACP synthase III (FabH) | yes | yes | 23 |
| PBANKA_0823800 | PF3D7_0922900 | 3-oxoacyl-[acyl-carrier-protein] reductase (FabG) | no | - | 23 |
| PY17X_0715100 | PF3D7_0815900 | dihydrolipoyl dehydrogenase, apicoplast (PDH E3) | no | - | |
| PBANKA_0923800 | PF3D7_1124500 | pyruvate dehydrogenase E1 component subunit | no | - | 73 |
| alpha (PDH E1α) | |||||
| PY17X_0934900 | PF3D7_1114800 | glycerol-3-phosphate dehydrogenase, putative (apiG3PDH) | no | - | 74 |
| PBANKA_0923800 | PF3D7_1114800 | glycerol-3-phosphate dehydrogenase, putative (apiG3PDH) | yes | yes | 73 |
| PY17X_1418400 | PF3D7_1318200 | glycerol-3-phosphate 1-O-acyltransferase (apiG3PAT) | no | - | 74 |
| PBANKA_0820900 | PF3D7_0920000 | long chain fatty acid elongation enzyme, putative (ELO3, ELO-A) | no | - | 23 |
| PBANKA_1357500 | PF3D7_1344600 | lipoyl synthase (LipA) | yes | yes | 23 |
| PBANKA_0707000 | PF3D7_0823600 | lipoate-protein ligase B (LipB) | yes | yes | 21 |
| PBANKA_1143400 | PF3D7_1367500 | NADH-cytochrome b5 reductase, putative (CBR) | yes | yes | 23 |
| PBANKA_0101100 | PF3D7_0602300 | liver merozoite formation protein (PALM) | Yes | Yes | 20 |
| PBANKA_0511000 | PF3D7_1026900 | biotin--protein ligase 1 (HCS1) | yes | yes | 21,23 |
| PY17X_1123700 | PF3D7_0623400 | MEI2-like RNA-binding protein (MEI2) | Yes | Yes | 24 |
| PBANKA_0214400 | PF3D7_0730300 | a transcription factor with AP2 domain(s) (AP2-L) | Yes | Yes | 75 |
| PBANKA_1024600 | PF3D7_1418100 | putative liver stage protein 1 (LISP1) | Yes | Yes | 76 |
| PBANKA_1003000 | PF3D7_0405300 | liver-specific protein 2 (LISP2; sequestrin) | Yes | Yes | 77,78 |
| PBANKA_0506500 | PF3D7_1022300 | ZIP domain-containing protein (ZIPCO) | Yes | Yes | 79 |
| PBANKA_0505000 | PF3D7_1020800 | dihydrolipoamide acyltransferase component E2 | yes | yes | 23 |
| PBANKA_1436200 | PF3D7_1221000 | histone-lysine N-methyltransferase, H3 lysine-4 specific | yes | yes | 23 |
| PBANKA_0304800 | PF3D7_0207400 | serine repeat antigen 7 | yes | yes | 80 |
| PBANKA_0404100 | PF3D7_0305600 | DNA-(apurinic or apyrimidinic site) endonuclease | yes | yes | 81 |
| PBANKA_1021500 | PF3D7_1421900 | copper transporter, putative (CTR2) | yes | yes | 82 |
Genes 1–7: Apicoplast-located proteins, involved in fatty acid synthesis (FAS II)–transformation of Acetyl-CoA into Acyl-ACP; Genes 8–12: Apicoplast-located proteins of the pyruvate dehydrogenase (PDH) complex, providing acetyl-CoA for fatty acid synthesis; Genes 13–17: (Putative) apicoplast-located proteins: not involved in the transformation of Acetyl-CoA into Acyl-ACP; Genes 16–19: genes selected for gene-deletion in P. falciparum.