Table 2.
Gametocyte, oocyst, and sporozoite production of four P. falciparum gene-deletion mutants and wild-type WT PfNF54.
| Lines | Stage V Gam. (%)1 | Exflag./105 RBC2 | Infection rate (%)3 | Oocyst no.4 | Spor. no (×103)6 |
|---|---|---|---|---|---|
| Average (s.d) | Average (s.d.) | Average (s.d.) | Average (s.d.) | Average (s.d.) | |
| WT PfNF54 | 2.04 (0.88) | 804 (387) | 66 (39) | 92 (86) | 53 (51) |
| 0.8–3.7 (11 exp.) | 201–1459 (27 exp.) | 68–100 (34 exp.) | 21–247 (34 exp.) | 4–155 (34 exp.) | |
| PfΔpalm | 2.85 (1.34) | 1544 (116) | 75 (18) | 38 (27) | 0 |
| 1.9–3.8 (2 exp.) | 1462–1626 (2 exp.) | 56–93 (4 exp.) | 19–64 (4 exp.) | (4 exp.) | |
| PfΔcbr | 1.6 (0.6) | 440 (219) | 53 (45) | 65 (52) | 0 |
| 0.8–2.1 (4 exp.) | 147–660 (9 exp.) | 54–100 (13 exp.) | 22–156 (13 exp.) | (11 exp.) | |
| PfΔhcs1 | 2.6 (0.8) | 459 (133) | 92(14) | 89 (42) | 0 |
| 1.8–3.3 (3 exp.) | 365–554 (2 exp.) | 76–100 (3 exp.) | 63–138 (3 exp.) | (3 exp.) | |
| PfΔmei2 | 2.1 (0.6) | 288 (182) | 73 (32) | 63 (68) | 31 (26) |
| 1.8–2.8 (3 exp.) | 94–600 (9 exp.) | 23–100 (14 exp.) | 14–167 (13 exp.) | 10–84 (9 exp.) |
1Mean percentage of blood-stage parasites developing into gametocytes in vivo.
2Mean percentage of exflagellating males in vitro, 12–15 minutes after activation.
3percentage of infected mosquitoes at days 7–12 after feeding.
4Mean number of oocysts per mosquito (days 7–12).
5Mean number of sporozoites per mosquito (days 21).
s.d.: standard deviation. Second raw: range and number of experiments.