Table 2.
UPLC gradient elution conditions.
| Time | Flow (mL/min) | A% | B% |
|---|---|---|---|
| Initial | 0.3 | 5 | 95 |
| 5 | 0.3 | 50 | 50 |
| 15 | 0.3 | 90 | 10 |
| 20 | 0.3 | 100 | 0 |
| 24 | 0.3 | 100 | 0 |
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