Fig. 4.

ASI-induced NED suppresses canonical AR signaling, cell cycle advancement, but not AR expression. (A-C) Heatmap of total cell population for DMSO and enzalutamide treated LNCaP cells showing enrichment for AR signaling score (B), direct transcription of AR gene itself (B), and non-canonical AR signaling (C). (D) Violin plot depicting AR expression at mRNA level for NEL C2 and C5. (E-F) DAVID enrichment of terms associated with more highly expressed gene signatures in NEL C2 (E) or NEL C5 (F). (G-I) TFs enriched in NEL C2 (G), NEL C5 (H), and CYCL C3/6/7/8 (I), separately.