Figure 4.

Differentially phosphorylated phosphoproteins and phosphosites in murine vs human skeletal muscle following exercise or maximal intensity contractions

(A) Venn diagram of differentially expressed phosphoproteins (DEpP) and differentially phosphorylated phosphosites (DPPS) that are unique and shared in mouse and human exercise datasets.

(B) UpSet plot comparing 4 mouse models of exercise and their DEpP and DPPS.

(C) Canonical pathways enriched in each exercise dataset.

(D) Scatterplot comparing the averaged mouse DPPS expression to the human exercise DPPS expression.

(E) Expression of DPPS in each mouse exercise dataset was compared to that of the human exercise dataset using Pearson’s correlation analysis. Significance cutoff for DEpP/DPPS was padj<0.05 for mouse data and padj<0.05 and expression fold change >|1.5| for human data. Significance for canonical pathways was-log(p value) ≥1.3 using a right-sided Fisher exact test. MIC = maximal intensity contraction, Treadmill (65% max.) exercise = mice exercised at 65% of their maximal running speed on a treadmill, Daytime exercise = mice that underwent high-intensity treadmill running during the zeitgeber time (ZT)0 period of “lights on,” Nighttime exercise = mice that underwent high-intensity treadmill running during the ZT12 period of “lights off.”