Fig. 3.

ER retention of missense α-Gal A mutants. (A) Representative immunofluorescence images showing the cellular distribution of wild type or mutant α-Gal A isoforms (red) and of the ER marker KDEL (green) in transiently transfected HEK293 GLA^−/− cells. (B) Quantification of α-Gal A (GLA) signal co-localizing with the ER marker KDEL (n = 20 cells). Data are shown as vertical scatterplots indicating mean ± s.d. (all mutant isoforms were compared to wild type, one-way ANOVA followed by Dunnett's multiple comparison test). **p < 0.01; *** p < 0.001.