Skip to main content
. 2022 Sep 26;298(11):102523. doi: 10.1016/j.jbc.2022.102523

Figure 3.

Figure 3

VPS35/VPS35 substructure reconstructions.A, VPS35/VPS35 substructure interface determined from retromer chains is shown with fitted model. B, VPS35/VPS35 substructure interface determined from retromer dimers of trimers (Fig. 2) is shown with fitted model. Coulomb potential maps generated using CCP4MG are shown at 4σ (A and B) contour levels. C and D, close-up views of VPS35/VPS35 dimer interfaces observed in flat chains (C) and dimers of trimers (D). Improvements in data processing resulted in reconstructions with higher resolution; these structures further support the presence of specific electrostatic residues (E615, D616, E617, K663, K701, and K705) mediating VPS35 dimer formation in asymmetric interfaces. Residues in the first dimer copy are labeled in black text, and residues in the second copy are labeled in black italic text.