Figure 5.

Analysis of retromer 3KE particle interfaces.A, schematic view of interactions observed in the retromer 3KE mutant particle. One heterotrimer is shown in dark colors (VPS29 in green, VPS35 in red, and VPS26 in blue), and the second heterotrimer is shown in transparent colors. Within each heterotrimer, N-VPS35 binds C-VPS26A (black and gray hatched circles). Each “chain link” structure is held together by an interaction between a VPS35 N terminus and the VPS26A N-terminal arrestin saddle in the neighboring heterotrimer (marked as black and gray circles). This interaction occurs on the opposite side from where the VPS35 N terminus interacts with a VPS26A subunit within its own heterotrimer. B, summary of buried surface area analysis between VPS35 and VPS26A (calculated using PISA, full details in text).