Skip to main content
. Author manuscript; available in PMC: 2022 Nov 5.
Published in final edited form as: Immunohorizons. 2022 Jan 14;6(1):8–15. doi: 10.4049/immunohorizons.2100113

FIGURE 4. Generation of protective NK cell memory responses by immunization in CC006 mice.

FIGURE 4.

(A) Experimental design. B6 and CC006 mice received i.v. transfer of either B6 splenocytes or B6-m157–expressing splenocytes, and no transfer mice served as an additional control. At day (D)3 and D5 posttransfer, mice that received splenocyte transfer were depleted of CD4 and CD8 T cells. Mice were bled at D7 to assess the impact of immunization on Ly49H NK cells. At D30 posttransfer, mice were infected with MCMV-Smith; a set of mice from each group were depleted of NK cells both 2 d prior to and on the day of infection. NK cell activation and viral titers were assessed at D33 posttransfer (D3 MCMV postinfection). (B–E) The number of Ly49H NK cells (B and C) and KLRG1+CD62L−Ly49H NK cells (D and E) in the spleen of CC006 (B and D) and B6 (C and E) mice 3 d after MCMV infection that were either naive, control immunized, or m157 immunized. (F) D3 liver MCMV titers in CC006 and B6 mice that were either naive, control immunized, or m157 immunized; infectious groups also included NK-depleted controls. Samples are representative of two independent experiments with four to eight mice per group. Error bars represent SEM. *p < 0.05.