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. 2022 Nov 5;15:163. doi: 10.1186/s13045-022-01376-3

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© The Author(s) 2022

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 7 — In vitro and in vivo on-target off-tumour effect of both CAR.CD123-T and CAR.CD123-NK cells against hematopoietic precursors. A Colony-forming unit (CFU) assay was performed with CD34⁺ cells derived from BM samples of 3 HDs after a short-term 4-h coculture with media alone (control condition, CNT; black line bars), un-transduced T cells (NT-T; black bars), CAR.CD123-T (green bars), un-transduced NK cells (NT-NK; blue bars) or CAR.CD123-NK cells (red bars). BFU-E and CFU-GM colonies were counted after 14 days. Results are shown as average of absolute count ± SD. B Schematic representation of humanized mouse model of hGM-CSF/hIL3 NOG (hu-NOG) mice engrafted with CD34⁺ cells derived from cord blood (CB) and infused with effector cells. C 15-Day probability of OS for humanized mouse model engrafted with adoptive transfer of NT-T (black line with circle), CAR.CD123-T (dotted green line with squares), NT-NK (blue line with rhombus) and CAR.CD123-NK (red line with triangles) cells. D Flow cytometry analysis of hCD45 cells in PB of hu-NOG mice before (grey bars) and (squared pattern bars) after 4 days from the effector cells infusion. Graphs show the percentage of hCD45⁺ cells on the total mononuclear cells present in PB (left panel), and the percentage of hCD123⁺ cells on the total hCD45⁺ cells present in the PB samples (right panel). Data are shown as average of ± SD. E–F Flow cytometry analysis of human hematopoietic cell quantification in tibia BM (E) and spleen (F) of hu-NOG mice receiving NT-T (black bars), CAR.CD123-T (green bars), NK-NT (blue bars) or CAR.CD123-NK (red bars). Graphs report the percentage of hCD45⁺ cells on the total mononuclear cells present in the investigated tissue (first panel), the percentage of hCD34⁻CD38⁺ cells on the total hCD45⁺ cells (second panel), the percentage of hCD34⁺CD38⁺ cells on the total hCD45⁺ cells (third panel), and the percentage of hCD34⁺CD38⁻ cells on the total hCD45.⁺ cells (fourth panel). Data are shown as average ± SD. *p < 0.05, **p < 0,001