FIG. 2.

Nucleotide sequence of the 2,245-bp insert from pGly1. Important restriction sites are underlined and labelled. The predicted amino acid sequence is shown below the DNA sequence. Putative promoter (−10 and −35) and ribosome binding sites (rbs) are double underlined. The transcriptional start point is the bold-faced A at position 708 and is indicated as +1. The amino-terminal 20-amino-acid residues determined by protein sequencing are shown in bold type. The boxed region of ORF2 denotes the region of homology with hemD (see text). Neisserial uptake sequences (15) are indicated by broken underline. This sequence has been deposited in GenBank with accession no. AF003941.