Fig. 3.
IL-6 induces RIG-I demethylation to enhance RIG-I-STAT3 association and feedback impede JAK2-STAT3 interaction. a RIG-I-STAT3 association induced by IL-6 was examined by immunoprecipitation in male mouse liver tissues. b IL-6-induced JAK2-STAT3 interaction was evaluated by immunoprecipitation in liver tissues from male Rig-If/f and Rig-Ihep−/− mice. c, d V5-tagged STAT3 and Flag-tagged RIG-I mutants as indicated were transfected into HHL5 hepatocyte cell line, and their association was tested by immunoprecipitation. e Methylated RIG-I at K18 and K146 were examined by the specific antibodies in the precipitates by total RIG-I antibody from the liver tissues upon IL-6 stimulation. f IL-6-induced STAT3 phosphorylation was evaluated in liver tissues from wildtype, RIG-I K18M+K146M or K18A+K146A mutant male mice upon IL-6 stimulation. g IL-6-induced RIG-I-STAT3 association and JAK2-STAT3 interaction were examined using immunoprecipitation in the liver tissues from male wildtype, RIG-I K18M+K146M or K18A+K146A mutant mice upon IL-6 stimulation. h Tumor incidence (chi-square test), number and maximum diameter (unpaired t-test) of DEN-induced HCC in male wildtype, RIG-I K18M+K146M or K18A+K146A mutant mice were analyzed (n = 12). Data are shown as mean ± s.d. or photographs from one representative of three independent experiments. *P < 0.05, **P < 0.01