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. 2022 Nov 4;19:269. doi: 10.1186/s12974-022-02633-5

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© The Author(s) 2022

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 2 — Fetuin-A is overexpressed after brain injury. A, B Western blot analysis and densitometric quantification of Fetuin-A expression by ImageJ in brain tissue of control (n = 3 samples) and TBI (n = 3 samples) patients. C, D Immunohistochemistry assessment of Fetuin-A expression in brain tissue from control and TBI patients. Scale bar is 20 or 100 μm. The relative intensity of Fetuin-A was detected with Image-J software. E The relative protein level of Fetuin-A in CCI6h vs. CCI24h (n = 8 samples) from quantitative proteomics analysis. F, G Protein levels of Fetuin-A obtained from sham mice and CCI mouse brain tissue. GAPDH is used as the loading control. And bar graphs show the results of analysis (by band density analysis) of Fetuin-A (sham = 3, CCI = 3). H, I Immunofluorescence assessment of Fetuin-A (green). Scale bar is 20 or 200 μm. The relative immunofluorescence intensity of Fetuin-A was detected with Image-J software. Data presented as mean ± SD (n = 5). *P < 0.05 vs. control group or sham group, **P < 0.01 vs. control group or sham group, and ^#P < 0.05 vs. CCI6h group