Fig. 4.

Dietary stress causes JNK-dependent cytoplasmic accumulation of RXRα. (A) WT mice and (B) Alb-Cre^+/− Mapk8^loxP/loxP Mapk9^loxP/loxP mice (age 8 wk) were fed a CD or a WD (16 wk). Liver sections were stained with an antibody to RXRα. DNA was stained with DAPI, and actin was stained with phalloidin. The sections were examined using a Leica SP8 confocal microscope. Representative images are presented. Scale bar, 20 µm. (C) The nuclear and cytoplasmic RXRα immunofluorescence was quantitated and presented as fluorescence intensity (mean ± SEM; ****P < 0.0001; not significant (NS), P > 0.05; n = 19–42 cells).