Fig. 6.

Enzymatic digestion of AEGISzyme C1 in native conditions. Eighteen percent, 7M urea PAGE. Left: time course of DNase I digestion. Right: time course of RNase H digestion. Time points are reported in minutes (‘). NR: nonreacted molecule. “DNA ladder”: 10-bp DNA ladder (New England Biolabs). “DNase I DNA ladder”: 10-bp DNA ladder digested with DNase I. Lengths in base pairs for the DNA ladders are in blue. RNA bases and sizes in nucleotides are indicated in black for ladders and DNA:RNA hybrid AEGISzyme C1. G8*: band resulting from the self-cleavage of C1 at its cleavage site during the digestion reaction, which is not a product of DNase I or RNase H digestion. U9**: hot spot for spontaneous hydrolysis of the RNA target, which is not a product of DNase I or RNase H digestion.