FIG. 1.
Effect of BisBAL and components on CPS expression. K. pneumoniae O1:K2 was cultured in defined medium for 18 h at 37°C with stirring at 200 rpm in the presence of different concentrations of bismuth nitrate (•), dimercaprol (■), or BisBAL (▴). CPS was extracted from whole cultures with a zwitterionic detergent in citric acid (9). Extract supernatants were ethanol precipitated and resolubilized in water. CPS was determined by measurement of its uronic acid content (2). Capsule expression is given as nanograms of uronic acid per 106 CFU. The data points represent the mean and standard deviation from at least three independent trials.