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. 2022 Oct 26;42(43):8169–8183. doi: 10.1523/JNEUROSCI.0401-22.2022

Figure 1.

Figure 1.

Generation of the AQP4-A25Q gene knocked-in mouse model. A, Schematic depicting the primary amino acid sequence of AQP4 and A25Q point mutation (left) and flowchart of the gene targeting strategy (right). B, Sequence analysis of the mutant alleles in AQP4A25Q/A25Q mice. The desired mutation “GCT” to “CAG” in the AQP4 gene was confirmed in knock-in mice. C, DNA sequence alignment of AQP4+/+ and AQP4A25Q/A25Q mice. D, Genotyping of AQP4+/+ and AQP4A25Q/A25Q mice. The size of the AQP4+/+ amplicon is 500 bp, and the size of the AQP4A25Q/A25Q amplicon is 540 bp. Primers used are provided in Extended Data Table 1-1.