Table 5.
The levels of AIF-1, miR-34a, ATG4B, and inflammatory factors in serum of db/db mice treatment with miR-34a agonist or antagonist for 12 weeks (n = 6).
| Parameters | db/db mice (12 weeks) | ||
|---|---|---|---|
| Control | miR-34a agonist | miR-34a antagonist | |
| Blood glucose (mmol/L) | 18.7 ± 5.24 | 20.8 ± 4.76 | 22.4 ± 6.18 |
| Total cholesterol (mmol/L) | 4.2 ± 1.03 | 4.7 ± 1.57 | 3.8 ± 1.43 |
| Triglycerides (mmol/L) | 2.7 ± 0.88 | 3.1 ± 1.08 | 3.5 ± 1.41 |
| Serum creatinine (mg/L) | 1.1 ± 0.46 | 1.4 ± 0.54∗ | 0.8 ± 0.39∗ |
| BUN (mmol/L) | 11.7 ± 3.17 | 13.2 ± 4.63 | 10.6 ± 5.11 |
| UACR (μg/mg) | 94.1 ± 16.19 | 110.6 ± 18.46# | 43.7 ± 13.82# |
| AST (unit/L) | 21.3 ± 4.32 | 24.6 ± 3.72 | 22.9 ± 4.48 |
| ALT (unit/L) | 42.6 ± 4.81 | 46.7 ± 4.66 | 41.2 ± 5.34 |
| Total protein (g/dL) | 4.2 ± 1.05 | 5.5 ± 1.45∗ | 2.5 ± 0.58# |
| AIF-1 (pg/mL) | 56.1 ± 6.51 | 60.1 ± 7.45∗ | 58.6 ± 6.29# |
| miR-34a (fold) | 4.1 ± 0.63 | 11.7 ± 1.86∗ | 0.3 ± 0.12# |
| NLRP3 (ng/ml) | 5.3 ± 1.24 | 19.6 ± 3.76∗ | 2.3 ± 0.54# |
| ATG4B (ng/ml) | 1.5 ± 0.41 | 0.2 ± 0.08∗ | 9.8 ± 2.23# |
| IL-1β (pg/mL) | 9.1 ± 1.55 | 38.1 ± 3.27∗ | 1.7 ± 0.26# |
| IL-18 (pg/mL) | 13.5 ± 1.98 | 53.7 ± 4.81∗ | 3.4 ± 0.44# |
BUN: urea nitrogen; UACR: urea albumin creatinine ratio; AST: aspartate aminotransferase; ALT: alanine transaminase; AIF-1: allograft inflammatory factor-1; miR-34a: microRNA 34a; ATG4B: autophagy related 4 homolog B; NLRP3: NOD-like receptor thermal protein domain associated protein 3; IL-1β: interleukin 1β; IL-18: interleukin 18; versus control, ∗P < 0.01, #P < 0.001.